Site-Specific Cryo-focused Ion Beam Sample Preparation Guided by 3D Correlative Microscopy

The development of cryo-focused ion beam (cryo-FIB) for the thinning of frozen-hydrated biological specimens enabled cryo-electron tomography (cryo-ET) analysis in unperturbed cells and tissues. However, the volume represented within a typical FIB lamella constitutes a small fraction of the biologic...

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發表在:Biophys J
Main Authors: Arnold, Jan, Mahamid, Julia, Lucic, Vladan, de Marco, Alex, Fernandez, Jose-Jesus, Laugks, Tim, Mayer, Tobias, Hyman, Anthony A., Baumeister, Wolfgang, Plitzko, Jürgen M.
格式: Artigo
語言:Inglês
出版: The Biophysical Society 2016
主題:
Cell Biophysics
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4775854/
https://ncbi.nlm.nih.gov/pubmed/26769364
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.10.053
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