Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography

Cryoelectron tomography provides unprecedented insights into the macromolecular and supramolecular organization of cells in a close-to-living state. However because of the limited thickness range (< 0.5–1 μm) that is accessible with today’s intermediate voltage electron microscopes only small pro...

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Bibliografiske detaljer
Main Authors: Rigort, Alexander, Bäuerlein, Felix J. B., Villa, Elizabeth, Eibauer, Matthias, Laugks, Tim, Baumeister, Wolfgang, Plitzko, Jürgen M.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2012
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3311327/
https://ncbi.nlm.nih.gov/pubmed/22392984
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1201333109
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