In situ Microfluidic Cryofixation for Cryo Focused Ion Beam Milling and Cryo Electron Tomography

We present a microfluidic platform for studying structure-function relationships at the cellular level by connecting video rate live cell imaging with in situ microfluidic cryofixation and cryo-electron tomography of near natively preserved, unstained specimens. Correlative light and electron micros...

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Bibliografische gegevens
Gepubliceerd in:Sci Rep
Hoofdauteurs: Fuest, Marie, Schaffer, Miroslava, Nocera, Giovanni Marco, Galilea-Kleinsteuber, Rodrigo I., Messling, Jan-Erik, Heymann, Michael, Plitzko, Jürgen M., Burg, Thomas P.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group UK 2019
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6911106/
https://ncbi.nlm.nih.gov/pubmed/31836773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-55413-2
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