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Cryo-focused Ion Beam Sample Preparation for Imaging Vitreous Cells by Cryo-electron Tomography

Cryo-electron tomography (CET) is a well-established technique for imaging cellular and molecular structures at sub-nanometer resolution. As the method is limited to samples that are thinner than 500 nm, suitable sample preparation is required to attain CET data from larger cell volumes. Recently, c...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Schaffer, Miroslava, Engel, Benjamin D., Laugks, Tim, Mahamid, Julia, Plitzko, Jürgen M., Baumeister, Wolfgang
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4900445/
https://ncbi.nlm.nih.gov/pubmed/27294174
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