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Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

Recent advances have made cryo-electron microscopy a key technique for achieving near-atomic resolution structures of biochemically isolated macromolecular complexes. Cryo-electron tomography (cryo-ET) can throw an unprecedented insight into these complexes in the context of their natural environmen...

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Dades bibliogràfiques
Publicat a:Nat Protoc
Autors principals: Wagner, Felix Roman, Watanabe, Reika, Schampers, Ruud, Singh, Digvijay, Persoon, Hans, Schaffer, Miroslava, Fruhstorfer, Peter, Plitzko, Jürgen, Villa, Elizabeth
Format: Artigo
Idioma:Inglês
Publicat: 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8053421/
https://ncbi.nlm.nih.gov/pubmed/32405053
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-020-0320-x
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