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Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography
Vitreous freezing offers a way to study cells and tissue in a near-native state by cryo-transmission electron microscopy (cryo-TEM), which is important when structural information at the macromolecular level is required. Many cells -- especially those in tissue -- are too thick to study intact in th...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3890102/ https://ncbi.nlm.nih.gov/pubmed/24211822 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2013.10.019 |
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