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Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam
Here we present a protocol used to prepare cryo-TEM samples of Aspergillus niger spores, but which can easily be adapted for any number of microorganisms or solutions. We make use of a custom built cryo-transfer station and a modified cryo-SEM preparation chamber(2). The spores are taken from a cult...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4511271/ https://ncbi.nlm.nih.gov/pubmed/25146386 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51463 |
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