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Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

One major limitation with current human embryonic stem cell (ESC) differentiation protocols is the generation of heterogeneous cell populations. These cultures contain the cells of interest, but are also contaminated with undifferentiated ESCs, non-neural derivatives and other neuronal subtypes.  Th...

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Pubblicato in:J Vis Exp
Autori principali: Hartley, Brigham J., Fabb, Stewart A., Finnin, Ben A.L., Haynes, John M., Pouton, Colin W.
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4758769/
https://ncbi.nlm.nih.gov/pubmed/25177806
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51764
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