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Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
One major limitation with current human embryonic stem cell (ESC) differentiation protocols is the generation of heterogeneous cell populations. These cultures contain the cells of interest, but are also contaminated with undifferentiated ESCs, non-neural derivatives and other neuronal subtypes. Th...
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Publicado no: | J Vis Exp |
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Main Authors: | , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
MyJove Corporation
2014
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4758769/ https://ncbi.nlm.nih.gov/pubmed/25177806 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51764 |
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