Selection-Independent Generation of Gene Knockout Mouse Embryonic Stem Cells Using Zinc-Finger Nucleases

Gene knockout in murine embryonic stem cells (ESCs) has been an invaluable tool to study gene function in vitro or to generate animal models with altered phenotypes. Gene targeting using standard techniques, however, is rather inefficient and typically does not exceed frequencies of 10(−6). In conse...

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Detalhes bibliográficos
Main Authors: Osiak, Anna, Radecke, Frank, Guhl, Eva, Radecke, Sarah, Dannemann, Nadine, Lütge, Fabienne, Glage, Silke, Rudolph, Cornelia, Cantz, Tobias, Schwarz, Klaus, Heilbronn, Regine, Cathomen, Toni
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3237556/
https://ncbi.nlm.nih.gov/pubmed/22194948
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0028911
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