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The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease

LAGLIDADG homing endonucleases (“meganucleases”) are highly specific DNA cleaving enzymes that are used for genome engineering. Like other enzymes that act on DNA targets, meganucleases often display binding affinities and cleavage activities that are dominated by one protein domain. To decipher the...

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Detalhes bibliográficos
Publicado no:J Mol Biol
Main Authors: Shen, Betty W., Lambert, Abigail, Walker, Bradley C., Stoddard, Barry L., Kaiser, Brett K.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4749321/
https://ncbi.nlm.nih.gov/pubmed/26705195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2015.12.005
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