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Engineering of customized meganucleases via in vitro compartmentalization and in cellulo optimization

LAGLIDADG homing endonucleases (also referred to as ‘meganucleases’) are compact DNA cleaving enzymes that specifically recognize long target sequences (approximately 20 base pairs), and thus serve as useful tools for therapeutic genome engineering. While stand-alone meganucleases are sufficiently a...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Takeuchi, Ryo, Choi, Michael, Stoddard, Barry L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4416406/
https://ncbi.nlm.nih.gov/pubmed/25408403
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-1862-1_6
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