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Engineering of customized meganucleases via in vitro compartmentalization and in cellulo optimization

LAGLIDADG homing endonucleases (also referred to as ‘meganucleases’) are compact DNA cleaving enzymes that specifically recognize long target sequences (approximately 20 base pairs), and thus serve as useful tools for therapeutic genome engineering. While stand-alone meganucleases are sufficiently a...

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Vydáno v:Methods Mol Biol
Hlavní autoři: Takeuchi, Ryo, Choi, Michael, Stoddard, Barry L.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2015
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4416406/
https://ncbi.nlm.nih.gov/pubmed/25408403
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-1862-1_6
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