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Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency
BACKGROUND: Single-guide RNA (sgRNA) is one of the two key components of the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 genome-editing system. The current commonly used sgRNA structure has a shortened duplex compared with the native bacterial CRISPR RNA (crRNA)–transacti...
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| Pubblicato in: | Genome Biol |
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| Autori principali: | , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
BioMed Central
2015
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4699467/ https://ncbi.nlm.nih.gov/pubmed/26671237 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0846-3 |
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