Chargement en cours...
Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans
The CRISPR RNA-guided Cas9 nuclease gene-targeting system has been successfully used for genome editing in a variety of organisms. Here, we report the use of dual sgRNA-guided Cas9 nuclease to generate knockout mutants of protein coding genes, noncoding genes, and repetitive sequences in C. elegans....
Enregistré dans:
| Publié dans: | Sci Rep |
|---|---|
| Auteurs principaux: | , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Nature Publishing Group
2014
|
| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4273605/ https://ncbi.nlm.nih.gov/pubmed/25531445 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep07581 |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|