Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency

BACKGROUND: Single-guide RNA (sgRNA) is one of the two key components of the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 genome-editing system. The current commonly used sgRNA structure has a shortened duplex compared with the native bacterial CRISPR RNA (crRNA)–transacti...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Genome Biol
Main Authors: Dang, Ying, Jia, Gengxiang, Choi, Jennie, Ma, Hongming, Anaya, Edgar, Ye, Chunting, Shankar, Premlata, Wu, Haoquan
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2015
Teme:
Research
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4699467/
https://ncbi.nlm.nih.gov/pubmed/26671237
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0846-3
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