Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila

The CRISPR/Cas9 system has recently emerged as a powerful tool for functional genomic studies in Drosophila melanogaster. However, sgRNA parameters affecting the specificity and efficiency of the system in flies are still not clear. Here, we found that off-target effects did not occur in regions of...

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Veröffentlicht in:Cell Rep
Hauptverfasser: Ren, Xingjie, Yang, Zhihao, Xu, Jiang, Sun, Jin, Mao, Decai, Hu, Yanhui, Yang, Su-Juan, Qiao, Huan-Huan, Wang, Xia, Hu, Qun, Deng, Patricia, Liu, Lu-Ping, Ji, Jun-Yuan, Li, Jin Billy, Ni, Jian-Quan
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2014
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4250831/
https://ncbi.nlm.nih.gov/pubmed/25437567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2014.09.044
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