Evaluation of CRISPR/Cas9 site-specific function and validation of sgRNA sequence by a Cas9/sgRNA-assisted reverse PCR technique

The effective application of the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system in biology, medicine and other fields is hindered by the off-target effects and loci-affinity of Cas9-sgRNA, especially at a genome-wide scale. In order to eliminate the occurrence of off-t...

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Detalhes bibliográficos
Publicado no:Anal Bioanal Chem
Main Authors: Zhang, Beibei, Zhou, Jiamu, Li, Miao, Wei, Yuanmeng, Wang, Jiaojiao, Wang, Yange, Shi, Pingling, Li, Xiaoli, Huang, Zixu, Tang, He, Song, Zongming
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Berlin Heidelberg 2021
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7929911/
https://ncbi.nlm.nih.gov/pubmed/33661348
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-021-03173-2
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