Enhanced Efficiency of flySAM by Optimization of sgRNA Parameters in Drosophila

The flySAM/CRISPRa system has recently emerged as a powerful tool for gain-of-function studies in Drosophila melanogaster. This system includes Gal4/UAS-driven dCas9 activators and U6 promoter-controlled sgRNA. Having established dCas9 activators superior to other combinations, to further enhance th...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:G3 (Bethesda)
Prif Awduron: Mao, Decai, Jia, Yu, Peng, Ping, Shen, Da, Ren, Xingjie, Zhu, Ruibao, Qiu, Yuhao, Han, Yuting, Yu, Jinchao, Che, Qinyun, Li, Yutong, Lu, Xinyi, Liu, Lu-Ping, Wang, Zhao, Liu, Qingfei, Sun, Jin, Ni, Jian-Quan
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Genetics Society of America 2020
Pynciau:
Investigations
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7718753/
https://ncbi.nlm.nih.gov/pubmed/33020192
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.120.401614
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