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Enhanced Efficiency of flySAM by Optimization of sgRNA Parameters in Drosophila
The flySAM/CRISPRa system has recently emerged as a powerful tool for gain-of-function studies in Drosophila melanogaster. This system includes Gal4/UAS-driven dCas9 activators and U6 promoter-controlled sgRNA. Having established dCas9 activators superior to other combinations, to further enhance th...
Αποθηκεύτηκε σε:
| Τόπος έκδοσης: | G3 (Bethesda) |
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| Κύριοι συγγραφείς: | , , , , , , , , , , , , , , , , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
Genetics Society of America
2020
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7718753/ https://ncbi.nlm.nih.gov/pubmed/33020192 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.120.401614 |
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