De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens

High-throughput CRISPR-Cas9 knockout screens using a tiling-sgRNA design permit in situ evaluation of protein domain function. Here, to facilitate de novo identification of essential protein domains from such screens, we propose ProTiler, a computational method for the robust mapping of CRISPR knock...

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Publicado en:Nat Commun
Main Authors: He, Wei, Zhang, Liang, Villarreal, Oscar D., Fu, Rongjie, Bedford, Ella, Dou, Jingzhuang, Patel, Anish Y., Bedford, Mark T., Shi, Xiaobing, Chen, Taiping, Bartholomew, Blaine, Xu, Han
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2019
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6778102/
https://ncbi.nlm.nih.gov/pubmed/31586052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-12489-8
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