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Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans
The CRISPR RNA-guided Cas9 nuclease gene-targeting system has been successfully used for genome editing in a variety of organisms. Here, we report the use of dual sgRNA-guided Cas9 nuclease to generate knockout mutants of protein coding genes, noncoding genes, and repetitive sequences in C. elegans....
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| Publicado en: | Sci Rep |
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| Autores principales: | , , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Nature Publishing Group
2014
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4273605/ https://ncbi.nlm.nih.gov/pubmed/25531445 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep07581 |
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