Target engagement and drug residence time can be observed in living cells with BRET

The therapeutic action of drugs is predicated on their physical engagement with cellular targets. Here we describe a broadly applicable method using bioluminescence resonance energy transfer (BRET) to reveal the binding characteristics of a drug with selected targets within intact cells. Cell-permea...

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Bibliografski detalji
Izdano u:Nat Commun
Glavni autori: Robers, Matthew B., Dart, Melanie L., Woodroofe, Carolyn C., Zimprich, Chad A., Kirkland, Thomas A., Machleidt, Thomas, Kupcho, Kevin R., Levin, Sergiy, Hartnett, James R., Zimmerman, Kristopher, Niles, Andrew L., Ohana, Rachel Friedman, Daniels, Danette L., Slater, Michael, Wood, Monika G., Cong, Mei, Cheng, Yi-Qiang, Wood, Keith V.
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2015
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Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4686764/
https://ncbi.nlm.nih.gov/pubmed/26631872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms10091
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