Utilizing a Simple Method for Stoichiometric Protein Labeling to Quantify Antibody Blockade

Ligand binding assays routinely employ fluorescently-labeled protein ligands to quantify the extent of binding. These ligands are commonly generated through chemical modification of accessible lysine residues, which often results in heterogeneous populations exhibiting variable binding properties. T...

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Udgivet i:Sci Rep
Main Authors: Friedman Ohana, Rachel, Hurst, Robin, Rosenblatt, Mike, Levin, Sergiy, Machleidt, Thomas, Kirkland, Thomas A., Encell, Lance P., Robers, Matthew B., Wood, Keith V.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2019
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6504924/
https://ncbi.nlm.nih.gov/pubmed/31065015
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-43469-z
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