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Utilizing a Simple Method for Stoichiometric Protein Labeling to Quantify Antibody Blockade

Ligand binding assays routinely employ fluorescently-labeled protein ligands to quantify the extent of binding. These ligands are commonly generated through chemical modification of accessible lysine residues, which often results in heterogeneous populations exhibiting variable binding properties. T...

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Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Friedman Ohana, Rachel, Hurst, Robin, Rosenblatt, Mike, Levin, Sergiy, Machleidt, Thomas, Kirkland, Thomas A., Encell, Lance P., Robers, Matthew B., Wood, Keith V.
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6504924/
https://ncbi.nlm.nih.gov/pubmed/31065015
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-43469-z
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