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Equilibrium and Kinetic Measurements of Ligand Binding to HiBiT-tagged GPCRs on the Surface of Living Cells

G-protein coupled receptors (GPCRs) remain at the forefront of drug discovery efforts. Detailed assessment of features contributing to GPCR ligand engagement in a physiologically relevant environment is imperative to the development of new therapeutics with improved efficacy. Traditionally, binding...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Boursier, Michelle E., Levin, Sergiy, Hurst, Robin, Ohana, Rachel Friedman
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842373/
https://ncbi.nlm.nih.gov/pubmed/33659503
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3861
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