Application of BRET to monitor ligand binding to GPCRs

Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a novel BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent...

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Publié dans:Nat Methods
Auteurs principaux: Stoddart, Leigh A, Johnstone, Elizabeth K M, Wheal, Amanda J, Goulding, Joëlle, Robers, Matthew B, Machleidt, Thomas, Wood, Keith V, Hill, Stephen J, Pfleger, Kevin D G
Format: Artigo
Langue:Inglês
Publié: 2015
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4488387/
https://ncbi.nlm.nih.gov/pubmed/26030448
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3398
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