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CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity
CRISPR/Cas9 has emerged as a versatile genome-engineering tool that relies on a single guide RNA (sgRNA) and the Cas9 enzyme for genome editing. Simple, fast and economical methods to generate sgRNAs have made targeted mutagenesis routine in cultured cells, mice, zebrafish and other model systems. P...
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| izdano v: | Nucleic Acids Res |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
2015
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4678847/ https://ncbi.nlm.nih.gov/pubmed/26253739 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv802 |
| Oznake: |
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