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CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity

CRISPR/Cas9 has emerged as a versatile genome-engineering tool that relies on a single guide RNA (sgRNA) and the Cas9 enzyme for genome editing. Simple, fast and economical methods to generate sgRNAs have made targeted mutagenesis routine in cultured cells, mice, zebrafish and other model systems. P...

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Bibliografiska uppgifter
I publikationen:Nucleic Acids Res
Huvudupphovsmän: Carrington, Blake, Varshney, Gaurav K., Burgess, Shawn M., Sood, Raman
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2015
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4678847/
https://ncbi.nlm.nih.gov/pubmed/26253739
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv802
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