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Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

The type II CRISPR/Cas system from Streptococcus pyogenes and its simplified derivative, the Cas9/single guide RNA (sgRNA) system, have emerged as potent new tools for targeted gene knockout in bacteria, yeast, fruit fly, zebrafish and human cells. Here, we describe adaptations of these systems lead...

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書目詳細資料
Main Authors: Jiang, Wenzhi, Zhou, Huanbin, Bi, Honghao, Fromm, Michael, Yang, Bing, Weeks, Donald P.
格式: Artigo
語言:Inglês
出版: Oxford University Press 2013
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3814374/
https://ncbi.nlm.nih.gov/pubmed/23999092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt780
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