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Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression

CRISPR interference (CRISPRi) represents a newly developed tool for targeted gene repression. It has great application potential for studying gene function and mapping gene regulatory elements. However, the optimal parameters for efficient single guide RNA (sgRNA) design for CRISPRi are not fully de...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Radzisheuskaya, Aliaksandra, Shlyueva, Daria, Müller, Iris, Helin, Kristian
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5062975/
https://ncbi.nlm.nih.gov/pubmed/27353328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw583
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