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Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression

CRISPR interference (CRISPRi) represents a newly developed tool for targeted gene repression. It has great application potential for studying gene function and mapping gene regulatory elements. However, the optimal parameters for efficient single guide RNA (sgRNA) design for CRISPRi are not fully de...

Полное описание

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Библиографические подробности
Опубликовано в: :Nucleic Acids Res
Главные авторы: Radzisheuskaya, Aliaksandra, Shlyueva, Daria, Müller, Iris, Helin, Kristian
Формат: Artigo
Язык:Inglês
Опубликовано: Oxford University Press 2016
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5062975/
https://ncbi.nlm.nih.gov/pubmed/27353328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw583
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