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Highly Specific Epigenome Editing by CRISPR/Cas9 Repressors for Silencing of Distal Regulatory Elements

Epigenome editing with the CRISPR/Cas9 platform is a promising technology to modulate gene expression to direct cell phenotype and to dissect the causal epigenetic mechanisms of gene regulation. Fusions of the nuclease-inactive dCas9 to the KRAB repressor (dCas9-KRAB) can silence target gene express...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Thakore, Pratiksha I., D’Ippolito, Anthony M, Song, Lingyun, Safi, Alexias, Shivakumar, Nishkala K., Kabadi, Ami M., Reddy, Timothy E., Crawford, Gregory E., Gersbach, Charles A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666778/
https://ncbi.nlm.nih.gov/pubmed/26501517
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3630
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