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RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors

CRISPR-Cas9 transcriptional repressors have emerged as robust tools for disrupting gene regulation in vitro but have not yet been adapted for systemic delivery in adult animal models. Here we describe a Staphylococcus aureus Cas9-based repressor (dSaCas9(KRAB)) compatible with adeno-associated viral...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Thakore, Pratiksha I., Kwon, Jennifer B., Nelson, Christopher E., Rouse, Douglas C., Gemberling, Matthew P., Oliver, Matthew L., Gersbach, Charles A.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5920046/
https://ncbi.nlm.nih.gov/pubmed/29700298
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-04048-4
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