Continuously Tunable Nucleic Acid Hybridization Probes

In silico designed nucleic acid probes and primers often fail to achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. Here, we present a novel, on-the-fly method of tuning probe af...

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Vydáno v:Nat Methods
Hlavní autoři: Wu, Lucia R., Wang, J. Sherry, Fang, John Z., Reiser, Emily, Pinto, Alessandro, Pekker, Irena, Boykin, Richard, Ngouenet, Celine, Webster, Philippa J., Beechem, Joseph, Zhang, David Yu
Médium: Artigo
Jazyk:Inglês
Vydáno: 2015
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666732/
https://ncbi.nlm.nih.gov/pubmed/26480474
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3626
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