Continuously Tunable Nucleic Acid Hybridization Probes

In silico designed nucleic acid probes and primers often fail to achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. Here, we present a novel, on-the-fly method of tuning probe af...

Descrición completa

Gardado en:
Detalles Bibliográficos
Publicado en:Nat Methods
Main Authors: Wu, Lucia R., Wang, J. Sherry, Fang, John Z., Reiser, Emily, Pinto, Alessandro, Pekker, Irena, Boykin, Richard, Ngouenet, Celine, Webster, Philippa J., Beechem, Joseph, Zhang, David Yu
Formato: Artigo
Idioma:Inglês
Publicado: 2015
Assuntos:
Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666732/
https://ncbi.nlm.nih.gov/pubmed/26480474
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3626
Tags: Engadir etiqueta
Sen Etiquetas, Sexa o primeiro en etiquetar este rexistro!

Títulos similares