Continuously Tunable Nucleic Acid Hybridization Probes

In silico designed nucleic acid probes and primers often fail to achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. Here, we present a novel, on-the-fly method of tuning probe af...

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Pubblicato in:Nat Methods
Autori principali: Wu, Lucia R., Wang, J. Sherry, Fang, John Z., Reiser, Emily, Pinto, Alessandro, Pekker, Irena, Boykin, Richard, Ngouenet, Celine, Webster, Philippa J., Beechem, Joseph, Zhang, David Yu
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2015
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666732/
https://ncbi.nlm.nih.gov/pubmed/26480474
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3626
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