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Continuously Tunable Nucleic Acid Hybridization Probes
In silico designed nucleic acid probes and primers often fail to achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. Here, we present a novel, on-the-fly method of tuning probe af...
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| Опубликовано в: : | Nat Methods |
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| Главные авторы: | , , , , , , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2015
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4666732/ https://ncbi.nlm.nih.gov/pubmed/26480474 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3626 |
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