Continuously Tunable Nucleic Acid Hybridization Probes

In silico designed nucleic acid probes and primers often fail to achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. Here, we present a novel, on-the-fly method of tuning probe af...

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Опубликовано в: :Nat Methods
Главные авторы: Wu, Lucia R., Wang, J. Sherry, Fang, John Z., Reiser, Emily, Pinto, Alessandro, Pekker, Irena, Boykin, Richard, Ngouenet, Celine, Webster, Philippa J., Beechem, Joseph, Zhang, David Yu
Формат: Artigo
Язык:Inglês
Опубликовано: 2015
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Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666732/
https://ncbi.nlm.nih.gov/pubmed/26480474
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3626
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