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Using a Split Luciferase Assay (SLA) to measure the kinetics of cell-cell fusion mediated by herpes simplex virus glycoproteins

Herpes simplex virus (HSV) entry and cell-cell fusion require the envelope proteins gD, gH/gL and gB. We propose that receptor-activated conformational changes to gD activate gH/gL, which then triggers gB (the fusogen) into an active form. To study this dynamic process, we have adapted a dual split...

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Publicat a:Methods
Autors principals: Saw, Wan Ting, Matsuda, Zene, Eisenberg, Roselyn J, Cohen, Gary H, Atanasiu, Doina
Format: Artigo
Idioma:Inglês
Publicat: 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4655157/
https://ncbi.nlm.nih.gov/pubmed/26022509
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2015.05.021
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