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Single-stranded DNA cleavage by divergent CRISPR-Cas9 enzymes
Double-stranded DNA (dsDNA) cleavage by Cas9 is a hallmark of type II CRISPR-Cas immune systems. Cas9–guide RNA complexes recognize 20-base-pair sequences in DNA and generate a site-specific double-strand break, a robust activity harnessed for genome editing. DNA recognition by all studied Cas9 enzy...
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| Izdano u: | Mol Cell |
|---|---|
| Glavni autori: | , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2015
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4636735/ https://ncbi.nlm.nih.gov/pubmed/26545076 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2015.10.030 |
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