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FRET-based cholesterolysis assay identifies a novel hedgehog inhibitor

Hedgehog (Hh) proteins function in cell/cell signaling processes linked to human embryo development and the progression of several types of cancer. Here we describe an optical assay of hedgehog cholesterolysis, a unique autoprocessing event critical for Hh function. The assay uses a recombinant FRET...

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Bibliografiska uppgifter
I publikationen:Anal Biochem
Huvudupphovsmän: Owen, Timothy S., Ngoje, George, Lageman, Travis J., Bordeau, Brandon M., Belfort, Marlene, Callahan, Brian P.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2015
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4591182/
https://ncbi.nlm.nih.gov/pubmed/26095399
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.06.021
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