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Chemical Bypass of General Base Catalysis in Hedgehog Protein Cholesterolysis Using a Hyper-Nucleophilic Substrate

Proteins in the hedgehog family undergo self-catalyzed endoproteolysis involving nucleophilic attack by a molecule of cholesterol. Recently, a conserved aspartate residue (D303, or D46) of hedgehog was identified as the general base that activates cholesterol during this unusual autoprocessing event...

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Bibliografiske detaljer
Udgivet i:J Am Chem Soc
Main Authors: Ciulla, Daniel A., Jorgensen, Michael T., Giner, José-Luis, Callahan, Brian P.
Format: Artigo
Sprog:Inglês
Udgivet: 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6054137/
https://ncbi.nlm.nih.gov/pubmed/28930454
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.7b05161
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