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Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes

Little is known about contacts in the spliceosome between proteins and intron nucleotides surrounding the pre-mRNA branch-site and their dynamics during splicing. We investigated protein-pre-mRNA interactions by UV-induced crosslinking of purified yeast B(act) spliceosomes formed on site-specificall...

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Библиографические подробности
Опубликовано в: :PLoS Genet
Главные авторы: Schneider, Cornelius, Agafonov, Dmitry E., Schmitzová, Jana, Hartmuth, Klaus, Fabrizio, Patrizia, Lührmann, Reinhard
Формат: Artigo
Язык:Inglês
Опубликовано: Public Library of Science 2015
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4579134/
https://ncbi.nlm.nih.gov/pubmed/26393790
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pgen.1005539
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