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Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS
The compositional and conformational changes during catalytic activation of the spliceosome promoted by the DEAH box ATPase Prp2 are only poorly understood. Here, we show by dual-color fluorescence cross-correlation spectroscopy (dcFCCS) that the binding affinity of several proteins is significantly...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Cold Spring Harbor Laboratory Press
2012
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3358646/ https://ncbi.nlm.nih.gov/pubmed/22535589 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.033316.112 |
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