Versatile genetic assembly system (VEGAS) to assemble pathways for expression in S. cerevisiae

We have developed a method for assembling genetic pathways for expression in Saccharomyces cerevisiae. Our pathway assembly method, called VEGAS (Versatile genetic assembly system), exploits the native capacity of S. cerevisiae to perform homologous recombination and efficiently join sequences with...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nucleic Acids Res
Prif Awduron: Mitchell, Leslie A., Chuang, James, Agmon, Neta, Khunsriraksakul, Chachrit, Phillips, Nick A., Cai, Yizhi, Truong, David M., Veerakumar, Ashan, Wang, Yuxuan, Mayorga, María, Blomquist, Paul, Sadda, Praneeth, Trueheart, Joshua, Boeke, Jef D.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2015
Pynciau:
Synthetic Biology and Bioengineering
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4513848/
https://ncbi.nlm.nih.gov/pubmed/25956652
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv466
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