Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs

Determining the mechanism of gene function is greatly enhanced using conditional mutagenesis. However, generating engineered conditional alleles is inefficient and has only been widely used in mice. Importantly, multiplex conditional mutagenesis requires extensive breeding. Here we demonstrate a sys...

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Veröffentlicht in:Genetics
Hauptverfasser: Yin, Linlin, Maddison, Lisette A., Li, Mingyu, Kara, Nergis, LaFave, Matthew C., Varshney, Gaurav K., Burgess, Shawn M., Patton, James G., Chen, Wenbiao
Format: Artigo
Sprache:Inglês
Veröffentlicht: Genetics Society of America 2015
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Investigations
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4492370/
https://ncbi.nlm.nih.gov/pubmed/25855067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.115.176917
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