Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs

Determining the mechanism of gene function is greatly enhanced using conditional mutagenesis. However, generating engineered conditional alleles is inefficient and has only been widely used in mice. Importantly, multiplex conditional mutagenesis requires extensive breeding. Here we demonstrate a sys...

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Dettagli Bibliografici
Pubblicato in:Genetics
Autori principali: Yin, Linlin, Maddison, Lisette A., Li, Mingyu, Kara, Nergis, LaFave, Matthew C., Varshney, Gaurav K., Burgess, Shawn M., Patton, James G., Chen, Wenbiao
Natura: Artigo
Lingua:Inglês
Pubblicazione: Genetics Society of America 2015
Soggetti:
Investigations
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4492370/
https://ncbi.nlm.nih.gov/pubmed/25855067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.115.176917
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