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Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow
A lack of techniques to image multiple genomic loci in living cells has limited our ability to investigate chromosome dynamics. Here we describe CRISPRainbow, a system for labeling DNA in living cells based on nuclease-dead (d) Cas9 combined with engineered single guide RNA (sgRNA) scaffolds that bi...
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| Publicado no: | Nat Biotechnol |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4864854/ https://ncbi.nlm.nih.gov/pubmed/27088723 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3526 |
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