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A High-Throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish

Zebrafish is a popular model organism for studying development and disease and genetically modified zebrafish provide an essential tool for functional genomic studies. Numerous publications have demonstrated the efficacy of gene targeting in zebrafish using CRISPR/Cas9, and have included description...

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Bibliografski detalji
Izdano u:Nat Protoc
Glavni autori: Varshney, Gaurav K., Carrington, Blake, Pei, Wuhong, Bishop, Kevin, Chen, Zelin, Fan, Chunxin, Xu, Lisha, Jones, MaryPat, LaFave, Matthew C., Ledin, Johan, Sood, Raman, Burgess, Shawn M.
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5630457/
https://ncbi.nlm.nih.gov/pubmed/27809318
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.141
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