High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9

The use of CRISPR/Cas9 as a genome-editing tool in various model organisms has radically changed targeted mutagenesis. Here, we present a high-throughput targeted mutagenesis pipeline using CRISPR/Cas9 technology in zebrafish that will make possible both saturation mutagenesis of the genome and larg...

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Detalhes bibliográficos
Publicado no:Genome Res
Main Authors: Varshney, Gaurav K., Pei, Wuhong, LaFave, Matthew C., Idol, Jennifer, Xu, Lisha, Gallardo, Viviana, Carrington, Blake, Bishop, Kevin, Jones, MaryPat, Li, Mingyu, Harper, Ursula, Huang, Sunny C., Prakash, Anupam, Chen, Wenbiao, Sood, Raman, Ledin, Johan, Burgess, Shawn M.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2015
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4484386/
https://ncbi.nlm.nih.gov/pubmed/26048245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.186379.114
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