High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9

The use of CRISPR/Cas9 as a genome-editing tool in various model organisms has radically changed targeted mutagenesis. Here, we present a high-throughput targeted mutagenesis pipeline using CRISPR/Cas9 technology in zebrafish that will make possible both saturation mutagenesis of the genome and larg...

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Dades bibliogràfiques
Publicat a:Genome Res
Autors principals: Varshney, Gaurav K., Pei, Wuhong, LaFave, Matthew C., Idol, Jennifer, Xu, Lisha, Gallardo, Viviana, Carrington, Blake, Bishop, Kevin, Jones, MaryPat, Li, Mingyu, Harper, Ursula, Huang, Sunny C., Prakash, Anupam, Chen, Wenbiao, Sood, Raman, Ledin, Johan, Burgess, Shawn M.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2015
Matèries:
Method
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4484386/
https://ncbi.nlm.nih.gov/pubmed/26048245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.186379.114
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