High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9

The use of CRISPR/Cas9 as a genome-editing tool in various model organisms has radically changed targeted mutagenesis. Here, we present a high-throughput targeted mutagenesis pipeline using CRISPR/Cas9 technology in zebrafish that will make possible both saturation mutagenesis of the genome and larg...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Genome Res
Prif Awduron: Varshney, Gaurav K., Pei, Wuhong, LaFave, Matthew C., Idol, Jennifer, Xu, Lisha, Gallardo, Viviana, Carrington, Blake, Bishop, Kevin, Jones, MaryPat, Li, Mingyu, Harper, Ursula, Huang, Sunny C., Prakash, Anupam, Chen, Wenbiao, Sood, Raman, Ledin, Johan, Burgess, Shawn M.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Cold Spring Harbor Laboratory Press 2015
Pynciau:
Method
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4484386/
https://ncbi.nlm.nih.gov/pubmed/26048245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.186379.114
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