High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9

The use of CRISPR/Cas9 as a genome-editing tool in various model organisms has radically changed targeted mutagenesis. Here, we present a high-throughput targeted mutagenesis pipeline using CRISPR/Cas9 technology in zebrafish that will make possible both saturation mutagenesis of the genome and larg...

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Detaylı Bibliyografya
Yayımlandı:Genome Res
Asıl Yazarlar: Varshney, Gaurav K., Pei, Wuhong, LaFave, Matthew C., Idol, Jennifer, Xu, Lisha, Gallardo, Viviana, Carrington, Blake, Bishop, Kevin, Jones, MaryPat, Li, Mingyu, Harper, Ursula, Huang, Sunny C., Prakash, Anupam, Chen, Wenbiao, Sood, Raman, Ledin, Johan, Burgess, Shawn M.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Cold Spring Harbor Laboratory Press 2015
Konular:
Method
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4484386/
https://ncbi.nlm.nih.gov/pubmed/26048245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.186379.114
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