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Physical characterization and in vitro biological impact of highly aggregated antibodies separated into size-enriched populations by fluorescence-activated cell sorting

An IgG2 monoclonal antibody (mAb) solution was subjected to stirring, generating high concentrations of nanometer and subvisible particles, which were then successfully size enriched into different size bins by low speed centrifugation or a combination of gravitational sedimentation and Fluorescence...

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Detalhes bibliográficos
Publicado no:J Pharm Sci
Main Authors: Telikepalli, Srivalli, Shinogle, Heather E., Thapa, Prem S., Kim, Jae Hyun, Deshpande, Meghana, Jawa, Vibha, Middaugh, C. Russell, Narhi, Linda O., Joubert, Marisa K., Volkin, David B.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4448733/
https://ncbi.nlm.nih.gov/pubmed/25753756
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jps.24379
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