Probing structurally altered and aggregated states of therapeutically relevant proteins using GroEL coupled to bio-layer interferometry

The ability of a GroEL-based bio-layer interferometry (BLI) assay to detect structurally altered and/or aggregated species of pharmaceutically relevant proteins is demonstrated. Assay development included optimizing biotinylated-GroEL immobilization to streptavidin biosensors, combined with biophysi...

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Detalhes bibliográficos
Publicado no:Protein Sci
Main Authors: Naik, Subhashchandra, Kumru, Ozan S, Cullom, Melissa, Telikepalli, Srivalli N, Lindboe, Elizabeth, Roop, Taylor L, Joshi, Sangeeta B, Amin, Divya, Gao, Phillip, Middaugh, C Russell, Volkin, David B, Fisher, Mark T
Formato: Artigo
Idioma:Inglês
Publicado em: BlackWell Publishing Ltd 2014
Assuntos:
Methods and Applications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4287005/
https://ncbi.nlm.nih.gov/pubmed/25043635
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2515
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