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Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
Amide hydrogen/deuterium exchange (ssHDX-MS) and side-chain photolytic labeling (ssPL-MS) followed by mass spectrometric analysis can be valuable for characterizing lyophilized formulations of protein therapeutics. Labeling followed by suitable proteolytic digestion allows the protein structure and...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4422116/ https://ncbi.nlm.nih.gov/pubmed/25938927 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52503 |
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