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Process and Formulation Effects on Protein Structure in Lyophilized Solids using Mass Spectrometric Methods

Myoglobin (Mb) was lyophilized in the absence (Mb-A) and presence (Mb-B) of sucrose in a pilot-scale lyophilizer with or without controlled ice nucleation. Cake morphology was characterized using scanning electron microscopy (SEM) and changes in protein structure were monitored using solid-state Fou...

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Publicat a:J Pharm Sci
Autors principals: Iyer, Lavanya K., Sacha, Gregory A., Moorthy, Balakrishnan S., Nail, Steven L., Topp, Elizabeth M.
Format: Artigo
Idioma:Inglês
Publicat: 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4846509/
https://ncbi.nlm.nih.gov/pubmed/27044943
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xphs.2016.02.033
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